Filter: AllWBIPIHCIF

WB analysis using 67685-1-Ig (same clone as 67685-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 67685-1-Ig (NF45 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67685-1-PBS in a different storage buffer formulation.
IP experiment of HeLa using 67685-1-Ig (same clone as 67685-1-PBS)
IP result of anti-NF45 (IP:67685-1-Ig, 4ug; Detection:67685-1-Ig 1:3000) with HeLa cells lysate 1320 ug. This data was developed using the same antibody clone with 67685-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 67685-1-Ig (same clone as 67685-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67685-1-Ig (NF45 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67685-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 67685-1-Ig (same clone as 67685-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67685-1-Ig (NF45 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67685-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 67685-1-Ig (same clone as 67685-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67685-1-Ig (NF45 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67685-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 67685-1-Ig (same clone as 67685-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67685-1-Ig (NF45 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67685-1-PBS in a different storage buffer formulation.
IF Staining of HepG2 using 67685-1-Ig (same clone as 67685-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NF45 antibody (67685-1-Ig, Clone: 1A3A2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67685-1-PBS in a different storage buffer formulation.

NF45 Monoclonal antibody, PBS Only

NF45 Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 67685-1-PBS

Clone No.1A3A2

Review Product

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

ILF2, NF45, PRO3063

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

67685-1-PBS targets NF45 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity	Human, mouse, rat
Host / Isotype	Mouse / IgG2b
Class	Monoclonal
Type	Antibody
Immunogen	NF45 fusion protein Ag30388 Predict reactive species
Full Name	interleukin enhancer binding factor 2, 45kDa
Calculated Molecular Weight	43 kDa
Observed Molecular Weight	40 kDa
GenBank Accession Number	BC000382
Gene Symbol	ILF2
Gene ID (NCBI)	3608
RRID	AB_2882878
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q12905
Storage Buffer	PBS only , pH 7.3
Storage Conditions	Store at -80°C.

Background Information

Interleukin-2 enhancer binding factor 2 (ILF2), also named as nuclear factor 45 (NF45), plays important roles in regulating interleukin-2 expression in mammals. It forms a heterodimeric complex with ILF3, which may regulate transcription of the IL2 gene during T-cell activation. It can also promote the formation of stable DNA-dependent protein kinase holoenzyme complexes on DNA [PMID:11739746,17129403].