NF2 Polyclonal antibody

NF2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 10659-1-AP

Synonyms

Neurofibromin-2, Neurofibromin 2, Moesin-ezrin-radixin-like protein, Merlin

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Validation Data Gallery

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  WB analysis using 10659-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10659-1-AP (NF2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 10659-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10659-1-AP (NF2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 10659-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10659-1-AP (NF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 10659-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 10659-1-AP (NF2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 10659-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10659-1-AP (NF2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human stomach using 10659-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10659-1-AP (NF2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: Jurkat cells, human kidney tissue, mouse kidney tissue, NIH/3T3 cells, rat kidney tissue
• Positive IHC detected in: human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

10659-1-AP targets NF2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NF2 fusion protein Ag1013
• Full Name: neurofibromin 2 (merlin)
• Calculated Molecular Weight: 70 kDa
• Observed Molecular Weight: 64-66 kDa
• GenBank Accession Number: BC007279
• Gene Symbol: NF2
• Gene ID (NCBI): 4771
• RRID: AB_2149815
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Neurofibromatosis 2 (NF2) tumor suppressor protein-merlin is involved in Hippo/SWH signaling pathway that associated with tumor suppression by restricting proliferation and promoting apoptosis. It has been reported that merlin can change to phosphorylation form which inactivates merlin tumor suppressive abilities at serine 518 induced by Rac effector p21-activated kinase 2. The mutation of the NF2 tumor suppressor gene may lead to nervous system tumors, mesotheliomas and metastatic tumors.

Publications

Species	Application	Title
human	WB	J Exp Clin Cancer Res; The histone lysine acetyltransferase KAT2B inhibits cholangiocarcinoma growth: evidence for interaction with SP1 to regulate NF2-YAP signaling; Authors - Wenbo Ma