NF-H/NF200 Polyclonal antibody, PBS Only

NF-H/NF200 Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

Cat no : 18934-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 18934-1-AP (same clone as 18934-1-PBS)

  Various tissues were subjected to SDS PAGE followed by western blot with 18934-1-AP (NF-H antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18934-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain tissue using 18934-1-AP (same clone as 18934-1-PBS)

  WB result of 18934-1-AP. This data was developed using the same antibody clone with 18934-1-PBS in a different storage buffer formulation.

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  WB analysis using 18934-1-AP (same clone as 18934-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 18934-1-AP (NF-H/NF200 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18934-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 18934-1-AP (same clone as 18934-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 18934-1-AP (NF-H/NF200 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18934-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 18934-1-AP (same clone as 18934-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 18934-1-AP (NF-H/NF200 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18934-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 18934-1-AP (same clone as 18934-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 18934-1-AP (NF-H antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18934-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 18934-1-AP (same clone as 18934-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 18934-1-AP (NF-H antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18934-1-PBS in a different storage buffer formulation.

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  IF Staining of rat brain using 18934-1-AP (same clone as 18934-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using NF-H/NF200 antibody (18934-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18934-1-PBS in a different storage buffer formulation.

• 

  IF Staining of rat brain using 18934-1-AP (same clone as 18934-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using NF-H/NF200 antibody (18934-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18934-1-PBS in a different storage buffer formulation.

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Product Information

18934-1-PBS targets NF-H/NF200 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NF-H/NF200 fusion protein Ag13517
• Full Name: neurofilament, heavy polypeptide
• Calculated Molecular Weight: 112 kDa
• Observed Molecular Weight: 200 kDa, 140-160 kDa
• GenBank Accession Number: BC014185
• Gene Symbol: NF-H
• Gene ID (NCBI): 4744
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

NEFH (NF200), also named as KIAA0845 and NFH, belongs to the intermediate filament family. It has an important function in mature axons that is not subserved by the two smaller NF proteins. Neurofilaments are the 10 nm intermediate filaments found specifically in neurons. They are a major component of the cell's cytoskeleton, and provide support for normal axonal radial growth. Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. The names given to the three major neurofilament subunits are based upon the apparent molecular weight of the mammalian subunits on SDS-PAGE: NF-L, 65-68 kDa; NF-M,145-160 kDa and NF-H, 200-220 kDa. This antibody can recognize both NEFH and NEFM.