NEUROD1 Monoclonal antibody, PBS Only

NEUROD1 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3E10F1

Cat no : 66691-1-PBS

Synonyms

NEUROD, Class A basic helix-loop-helix protein 3, bHLHa3, 3E10F1

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Validation Data Gallery

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  WB analysis of Y79 using 66691-1-Ig (same clone as 66691-1-PBS)

  Y79 cells were subjected to SDS PAGE followed by western blot with 66691-1-Ig (NEUROD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66691-1-PBS in a different storage buffer formulation.

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  WB analysis of SH-SY5Y using 66691-1-Ig (same clone as 66691-1-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 66691-1-Ig (NEUROD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66691-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 66691-1-Ig (same clone as 66691-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66691-1-Ig (NEUROD1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66691-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 66691-1-Ig (same clone as 66691-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66691-1-Ig (NEUROD1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66691-1-PBS in a different storage buffer formulation.

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  FC experiment of SH-SY5Y using 66691-1-Ig (same clone as 66691-1-PBS)

  1X10^6 SH-SY5Y cells were intracellularly stained with 0.5 ug Anti-Human NEUROD1 (66691-1-Ig, Clone:3E10F1) (red) labeled with FlexAble CoraLite® Plus 555 Antibody Labeling Kit for Mouse IgG1 (KFA022), or 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66691-1-PBS in a different storage buffer formulation.

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Product Information

66691-1-PBS targets NEUROD1 in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: NEUROD1 fusion protein Ag27606
• Full Name: neurogenic differentiation 1
• Calculated Molecular Weight: 356 aa, 40 kDa
• Observed Molecular Weight: 40-50 kDa
• GenBank Accession Number: BC009046
• Gene Symbol: NEUROD1
• Gene ID (NCBI): 4760
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

NeuroD is a member of the basic helix-loop-helix (bHLH) family of transcription factors. The basic helix-loop-helix (bHLH) proteins are transcription factors that are required for several aspects of development, including cell type determination, terminal differentiation and sex determination. Members of the myogenic determination family, MyoD, myf5, myogenin and MRF4, all have bHLH domains.These proteins function by forming heterodimers with E-proteins and binding to the canonical E-box sequence CANNTG. Neuro D is expressed transiently in a subset of neurons in the central and peripheral nervous systems at the time of their terminal differentiation into mature neurons. Moreover, ectopic expression of Neuro D in Xenopus embryos induces premature differentiation of neuronal precursors and Neuro D can convert presumptive epidermal cells into neurons.The lack of NeuroD in the brain results in severe defects in development. Human mutations have been linked to a number of types of diabetes including type I diabetes mellitus and maturity-onset diabetes of the young. The calculated molecular weight of NEUROD1 is 39 kDa, but the modified NEUROD1 protein is about 45-50 kDa.