NEURL Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

NEURL Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 18898-1-PBS

Synonyms

neu, h-neuralized 1, h neuralized 1, h neu, EC:2.3.2.27

---

Validation Data Gallery

• 

  WB analysis of mouse brain using 18898-1-AP (same clone as 18898-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 18898-1-AP (NEURL antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18898-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 18898-1-AP (same clone as 18898-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 18898-1-AP (NEURL antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18898-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 18898-1-AP (same clone as 18898-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 18898-1-AP (NEURL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18898-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Y79 using 18898-1-AP (same clone as 18898-1-PBS)

  Y79 cells were subjected to SDS PAGE followed by western blot with 18898-1-AP (NEURL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18898-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse brain using 18898-1-AP (same clone as 18898-1-PBS)

  IP result of anti-NEURL (IP:18898-1-AP, 4ug; Detection:18898-1-AP 1:600) with mouse brain tissue lysate 2560ug. This data was developed using the same antibody clone with 18898-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 18898-1-AP (same clone as 18898-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18898-1-AP (NEURL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18898-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 18898-1-AP (same clone as 18898-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18898-1-AP (NEURL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18898-1-PBS in a different storage buffer formulation.

• 

  IF Staining of MCF-7 using 18898-1-AP (same clone as 18898-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using NEURL antibody (18898-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 18898-1-PBS in a different storage buffer formulation.

---

Product Information

18898-1-PBS targets NEURL in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NEURL fusion protein Ag13531
• Full Name: neuralized homolog (Drosophila)
• Calculated Molecular Weight: 59 kDa
• Observed Molecular Weight: 60-62 kDa
• GenBank Accession Number: BC026336
• Gene Symbol: NEURL
• Gene ID (NCBI): 9148
• RRID: AB_10642557
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

NEURL(Neuralized-like protein 1A) is also named as NEURL1, NEURL1A, RNF67.Neurl1 is a RING-dependent E3 ubiquitin ligase that can regulate Notch signaling in mammals and it expression promotes lysosomal degradation of Jagged1 in cells and blocks signaling from Jagged1-expressing cells to neighboring Notch-expressing cells. Neurl-dependent turnover of Jagged1 and inhibition of Notch signaling depends upon N-terminal myristoylation and plasma membrane localization of Neurl1(PMID:18077452).This antibody is specific to NEURL.