NEU3 Monoclonal antibody

• Featured Product
• KD/KO Validated

NEU3 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2C9C5

Cat no : 67098-1-Ig

Synonyms

cDNA FLJ78026, FLJ12388, NEU3, NEU3 protein, SIAL3

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Validation Data Gallery

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  WB analysis of PC-3 using 67098-1-Ig

  WB result of NEU3 antibody (67098-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NEU3 transfected PC-3 cells.

• 

  WB analysis using 67098-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67098-1-Ig (NEU3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 67098-1-Ig

  PC-3 cells were subjected to SDS PAGE followed by western blot with 67098-1-Ig (NEU3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 67098-1-Ig

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 67098-1-Ig (NEU3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-29 using 67098-1-Ig

  HT-29 cells were subjected to SDS PAGE followed by western blot with 67098-1-Ig (NEU3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 67098-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 67098-1-Ig (NEU3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of THP-1 using 67098-1-Ig

  THP-1 cells were subjected to SDS PAGE followed by western blot with 67098-1-Ig (NEU3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 67098-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 67098-1-Ig (NEU3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 67098-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67098-1-Ig (NEU3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 67098-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67098-1-Ig (NEU3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 67098-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67098-1-Ig (NEU3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: PC-3 cells, COLO 320 cells, HT-29 cells, K-562 cells, THP-1 cells, Caco-2 cells
• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67098-1-Ig targets NEU3 in WB, IHC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: NEU3 fusion protein Ag27272
• Full Name: sialidase 3 (membrane sialidase)
• Calculated Molecular Weight: 48 kDa
• Observed Molecular Weight: 48-52 kDa
• GenBank Accession Number: NM_006656.5
• Gene Symbol: NEU3
• Gene ID (NCBI): 10825
• RRID: AB_2882403
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.