NETO2 Recombinant antibody

• 

  WB analysis using 84222-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84222-2-RR (NETO2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  Affinity and Kinetic Characterization of 84222-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84222-2-RR against Human NETO2 were performed. The affinity constant is 0.203 nM.

NETO2 {ptg:Brand} Recombinant Antibody for WB, ELISA

Cat No. 84222-2-RR

Clone No.241451E10

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

UNQ1926/PRO4401, Neuropilin and tolloid-like protein 2, NEOT2, BTCL2, Brain-specific transmembrane protein containing 2 CUB and 1 LDL-receptor class A domains protein 2

---

Tested Applications

• Positive WB detected in: rat brain tissue, mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84222-2-RR targets NETO2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: NETO2 fusion protein Ag2971
• Full Name: neuropilin (NRP) and tolloid (TLL)-like 2
• Calculated Molecular Weight: 525 aa, 59 kDa
• Observed Molecular Weight: 59 kDa
• GenBank Accession Number: BC012381
• Gene Symbol: NETO2
• Gene ID (NCBI): 81831
• RRID: AB_3671776
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q8NC67
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Neuropilin and tolloid-like 2 (NETO2), a member of the subfamily of CUB domain and LDLa-containing proteins, was identified as an auxiliary protein of neuronal kainate receptors (KARs) and played critical roles in regulating the functions of KARs. It was also able to bind to the active oligomeric form of K+-Cl− cotransporter (KCC2) to enhance its recycling in hippocampal neurons. Recently, elevated mRNA levels of NETO2 were detected in several types of tumors.