NET1 Polyclonal antibody

NET1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 12740-1-AP

Synonyms

Proto-oncogene p65 Net1, Proto oncogene p65 Net1, Neuroepithelial cell-transforming gene 1 protein, NET1A, ARHGEF8

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Validation Data Gallery

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  WB analysis of Y79 using 12740-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 12740-1-AP (NET1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach using 12740-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 12740-1-AP (NET1 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human stomach using 12740-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 12740-1-AP (NET1 antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of A431 using 12740-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using NET1 antibody (12740-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: Y79 cells
• Positive IHC detected in: human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below

Product Information

12740-1-AP targets NET1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NET1 fusion protein Ag3636
• Full Name: neuroepithelial cell transforming 1
• Calculated Molecular Weight: 596 aa, 68 kDa
• Observed Molecular Weight: 68 kDa
• GenBank Accession Number: BC010285
• Gene Symbol: NET1
• Gene ID (NCBI): 10276
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IHC	Hum Pathol; Short interfering RNA targeting Net1 reduces the angiogenesis and tumor growth of in vivo cervical squamous cell carcinoma through VEGF down-regulation.; Authors - Yuting Zhang