Neudesin/NENF Polyclonal antibody

Neudesin/NENF Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 15760-1-AP

Synonyms

Neudesin / NENF, Neudesin, NENF, Protein GIG47, Neuron-derived neurotrophic factor

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Validation Data Gallery

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  WB analysis using 15760-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15760-1-AP (Neudesin/NENF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HCT 116 cells, HEK-293 cells, HeLa cells, MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15760-1-AP targets Neudesin/NENF in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Neudesin/NENF fusion protein Ag8387
• Full Name: neuron derived neurotrophic factor
• Calculated Molecular Weight: 172 aa, 19 kDa
• Observed Molecular Weight: 19 kDa
• GenBank Accession Number: BC008823
• Gene Symbol: NENF
• Gene ID (NCBI): 29937
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Neudesin neurotrophic factor (NENF, also known as CIR2, SPUF, and SCIRP10) acts as a neurotrophic factor in postnatal mature neurons enhancing neuronal survival, which is localized to mitochondria and endoplasmic reticulum by PINK1 and PARK7 (PMID: 31536960). NENF in the adult brain is expected to play roles in the maintenance and protection of neurons in an autocrine/paracrine manner (PMID: 15605373). It greatly increases cAMP levels in neural precursor cells and might activate a Gs-protein-coupled receptor that could activate the MAPK, PKA, and PI-3K signal pathways (PMID: 16547973).