NELF Polyclonal antibody

NELF Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 12244-1-AP

Synonyms

NSMF, NMDA receptor synaptonuclear signaling and neuronal migration factor, Nasal embryonic luteinizing hormone-releasing hormone factor, Nasal embryonic LHRH factor

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Validation Data Gallery

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  WB analysis of HeLa using 12244-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12244-1-AP (NELF Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human colon using 12244-1-AP

  human colon tissue were subjected to SDS PAGE followed by western blot with 12244-1-AP (NELF antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12244-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12244-1-AP (NELF antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 12244-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12244-1-AP (NELF antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 12244-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12244-1-AP (NELF antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 12244-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12244-1-AP (NELF Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 12244-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 12244-1-AP (NELF antibody) at dilution of 1:100 (under 10x lens).

• 

  IF Staining of HeLa using 12244-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 12244-1-AP (NELF antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, human brain tissue, human colon tissue, mouse testis tissue
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12244-1-AP targets NELF in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NELF fusion protein Ag2888
• Full Name: nasal embryonic LHRH factor
• Calculated Molecular Weight: 507 aa, 56 kDa
• Observed Molecular Weight: 54-60 kDa
• GenBank Accession Number: BC016862
• Gene Symbol: NELF
• Gene ID (NCBI): 26012
• RRID: AB_2150819
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NSMF, also named as NELF (Nasal embryonic LHRH factor), couples NMDA-sensitive glutamate receptor signaling to the nucleus and triggers long-lasting changes in the cytoarchitecture of dendrites and spine synapse processes. NSMF has some isoforms with MW 44 kDa and 55-60 kDa. This antibody recognizes all the isoforms.