NELF-A Polyclonal antibody

• Featured Product
• KD/KO Validated

NELF-A Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

5

Reviews (2)

Cat no : 10456-1-AP

Synonyms

WHSC2, Wolf-Hirschhorn syndrome candidate 2 protein, NELFA, NELF A

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Validation Data Gallery

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  WB analysis of HeLa using 10456-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10456-1-AP (NELF-A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 10456-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10456-1-AP (NELF-A antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 10456-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10456-1-AP (NELF-A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10456-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10456-1-AP (NELF-A Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10456-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10456-1-AP (NELF-A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 10456-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10456-1-AP (NELF-A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 10456-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10456-1-AP (NELF-A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 10456-1-AP

  IP result of anti-NELF-A (IP:10456-1-AP, 4ug; Detection:10456-1-AP 1:500) with mouse brain tissue lysate 3440ug.

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  IF Staining of HeLa using 10456-1-AP

  Immunofluorescent analysis of HeLa cells using 10456-1-AP (NELF-A antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, mouse liver tissue, Jurkat cells, mouse brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 4 publications below

Product Information

10456-1-AP targets NELF-A in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NELF-A fusion protein Ag0708
• Full Name: Wolf-Hirschhorn syndrome candidate 2
• Calculated Molecular Weight: 57 kDa
• Observed Molecular Weight: 57 kDa
• GenBank Accession Number: BC002764
• Gene Symbol: NELFA
• Gene ID (NCBI): 7469
• RRID: AB_2216327
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

WHSC2, also known as NELF-A, is a component of the Negative ELongation Factor complex, which inhibits RNA polymerase II progression early during elongation (a process referred to as 'promoter proximal pausing'), thereby altering expression of its target genes in a positive and negative manner [PMID:19245807]. This complex acts with DRB sensitivity-inducing factor (DSIF), a heterodimer of SPT4 and SPT5, to cause transcriptional pausing of RNA polymerase II[PMID:12612062].

Publications

Species	Application	Title
		Mol Cell; SPT5 stabilization of promoter-proximal RNA polymerase II.; Authors - Yuki Aoi
mouse	WB	Nat Commun; RNA polymerase II pausing factor NELF in CD8+ T cells promotes antitumor immunity.; Authors - Bogang Wu
human	WB	Nucleic Acids Res; Multiple P-TEFbs cooperatively regulate the release of promoter-proximally paused RNA polymerase II.; Authors - Xiaodong Lu ; KD Validated
human	WB	Nat Commun; ERK-mediated NELF-A phosphorylation promotes transcription elongation of immediate-early genes by releasing promoter-proximal pausing of RNA polymerase II; Authors - Seina Ohe ; KD Validated
human	WB	J Biol Chem; An Essential Signaling Function of Cytoplasmic NELFB is Independent of RNA Polymerase II Pausing; Authors - Haihui Pan

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Huai-Chin (Verified Customer) (06-29-2023)

Decent antibody with major bands at expected size. There are some non-specific weaker bands.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse T cells isolated from spleen

FH

Carly (Verified Customer) (09-27-2019)

Used in an antibody array to identify proteins in human EDTA plasma.

• Applications: Other,