NEK4 Recombinant antibody

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  WB analysis using 84654-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84654-4-RR (NEK4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 84654-4-RR

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 84654-4-RR (NEK4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 84654-4-RR

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 84654-4-RR (NEK4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 84654-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84654-4-RR against Human NEK4 were performed. The affinity constant is 92.8 pM.

NEK4 Recombinant Antibody for WB, IHC, ELISA

Cat No. 84654-4-RR

Clone No.242081B9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

STK2, pp12301, NRK2, NimA related protein kinase 4, 242081B9

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Tested Applications

• Positive WB detected in: A549 cells, HEK-293T cells, HeLa cells, NCI-H1299 cells
• Positive IHC detected in: mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84654-4-RR targets NEK4 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: NEK4 fusion protein Ag34929
• Full Name: NIMA (never in mitosis gene a)-related kinase 4
• Calculated Molecular Weight: 781 aa, 88 kDa
• Observed Molecular Weight: 95-100 kDa
• GenBank Accession Number: BC063044
• Gene Symbol: NEK4
• Gene ID (NCBI): 6787
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P51957
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Neks (NIMA-related kinases) are a group of serine-threonine kinases that are related to NIMA, their ortholog from Aspergillus nidulans, which is essential for cells to enter in mitosis. NEK4 was initially identified as STK2, from serine/threonine kinase 2, in a study using a kinase specific cDNA library from human breast cancer tumors or breast cancer cells.