NEDD4 Recombinant antibody

NEDD4 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

CloneNo.

230478F1

Cat no : 83112-1-RR

Synonyms

KIAA0093, HECT-type E3 ubiquitin transferase NEDD4, EC:2.3.2.26, E3 ubiquitin-protein ligase NEDD4, Cell proliferation-inducing gene 53 protein

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Validation Data Gallery

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  WB analysis using 83112-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83112-1-RR (NEDD4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IP experiment of NIH/3T3 using 83112-1-RR

  IP result of anti-NEDD4 (IP:83112-1-RR, 4ug; Detection:83112-1-RR 1:1000) with NIH/3T3 cells lysate 1505 ug.

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  IF Staining of U2OS using 83112-1-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using NEDD4 antibody (83112-1-RR, Clone: 230478F1 ) at dilution of 1:100 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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  Affinity and Kinetic Characterization of 83112-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83112-1-RR against Human NEDD4 were performed. The affinity constant is 14.8 nM.

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Tested Applications

• Positive WB detected in: C6 cells, NIH/3T3 cells
• Positive IP detected in: NIH/3T3 cells
• Positive IF/ICC detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83112-1-RR targets NEDD4 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: NEDD4 fusion protein Ag16291
• Full Name: neural precursor cell expressed, developmentally down-regulated 4
• Calculated Molecular Weight: 1319 aa, 149 kDa
• Observed Molecular Weight: 115 kDa
• GenBank Accession Number: BC152452
• Gene Symbol: NEDD4
• Gene ID (NCBI): 4734
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NEDD4 and similar proteins discovered subsequently became a family of HECT ligases, comprising 9 human proteins including NEDD4, NEDD4-2 (NEDD4L), ITCH, SMURF1, SMURF2, WWP1, WWP2, NEDL1 and NEDL2. NEDD4 is a highly evolutionarily conserved protein from yeast to man, and was initially cloned as a highly expressed gene in the early embryonic brain. NEDD4 is frequently overexpressed in many different types of cancer, and decreased levels of NEDD4 can also be associated with cancer. It can be a potential therapeutic target for the treatment of human cancer.(PMID: 25527121). The human NEDD4 gene is located on chromosome 15q21.3 and comprises 30 exons (HGNC:7727) shown to encode a ~120 KDa protein. Otherwise there is a 75 kDa isoform in addition to full length NEDD4 (PMID: 24907641).