NECAP1 Polyclonal antibody, PBS Only

NECAP1 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 17906-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 17906-1-AP (same clone as 17906-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 17906-1-AP (NECAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17906-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 17906-1-AP (same clone as 17906-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 17906-1-AP (NECAP1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17906-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 17906-1-AP (same clone as 17906-1-PBS)

  IP result of anti-NECAP1 (IP:17906-1-AP, 4ug; Detection:17906-1-AP 1:300) with mouse brain tissue lysate 6000 ug. This data was developed using the same antibody clone with 17906-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 17906-1-AP (same clone as 17906-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17906-1-AP (NECAP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17906-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 17906-1-AP (same clone as 17906-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17906-1-AP (NECAP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17906-1-PBS in a different storage buffer formulation.

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Product Information

17906-1-PBS targets NECAP1 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NECAP1 fusion protein Ag12283
• Full Name: NECAP endocytosis associated 1
• Calculated Molecular Weight: 275 aa, 30 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC110876
• Gene Symbol: NECAP1
• Gene ID (NCBI): 25977
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.