NEBL Polyclonal antibody

NEBL Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 21497-1-AP

Synonyms

Nebulette, LNEBL, C10orf113, Actin-binding Z-disk protein, Actin binding Z disk protein

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Validation Data Gallery

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  WB analysis using 21497-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21497-1-AP (NEBL antibody) at dilution of 1:60000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse heart using 21497-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 21497-1-AP (NEBL antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 21497-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 21497-1-AP (NEBL antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse heart using 21497-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using NEBL antibody (21497-1-AP) at dilution of 1:600 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse heart tissue, rat heart tissue
• Positive IHC detected in: mouse heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse heart tissue

Recommended dilution

• Western Blot (WB): WB : 1:20000-1:100000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)-P: IF-P : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

21497-1-AP targets NEBL in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NEBL fusion protein Ag15882
• Full Name: nebulette
• Calculated Molecular Weight: 1014 aa, 116 kDa
• Observed Molecular Weight: 116 kDa
• GenBank Accession Number: BC110453
• Gene Symbol: NEBL
• Gene ID (NCBI): 10529
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Nebulette, also known as NEBL, is a cardiac-specific isoform belonging to the nebulin family of proteins. Nebulette has 23 modular repeat structures of 35 amino acid residues each. Nebulette has an acidic region with unknown structure at its N-terminus, and a serine-rich region adjacent to an SH3 domain at its C-terminus (PMID: 2037050, 20951588). Nebulette functionally links sarcomeric actin to the desmin intermediate filaments in the heart muscle sarcomeres (PubMed:27733623). Nebulette is abundantly expressed in cardiac muscle at Z-disc regions, but not in skeletal or smooth muscle.