NDUFS5 Monoclonal antibody, PBS Only (Detector)

NDUFS5 Monoclonal Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2C2A5

Cat no : 66053-1-PBS

Synonyms

NADH dehydrogenase [ubiquinone] iron-sulfur protein 5, Complex I-15 kDa, Complex I 15 kDa, CI-15 kDa, CI 15 kDa

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Validation Data Gallery

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  WB analysis of HepG2 using 66053-1-Ig (same clone as 66053-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66053-1-Ig (NDUFS5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66053-1-PBS in a different storage buffer formulation.

• 

  WB analysis of L02 using 66053-1-Ig (same clone as 66053-1-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 66053-1-Ig (NDUFS5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66053-1-PBS in a different storage buffer formulation.

• 

  WB analysis of fetal human brain using 66053-1-Ig (same clone as 66053-1-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66053-1-Ig (NDUFS5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66053-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 66053-1-Ig (same clone as 66053-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66053-1-Ig (NDUFS5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66053-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 66053-1-Ig (same clone as 66053-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 66053-1-Ig(NDUFS5 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66053-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 66053-1-Ig (same clone as 66053-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 66053-1-Ig(NDUFS5 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66053-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 66053-1-Ig (same clone as 66053-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 66053-1-Ig(NDUFS5 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66053-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 66053-1-Ig (same clone as 66053-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 66053-1-Ig(NDUFS5 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66053-1-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP50607-1

  Cytometric bead array standard curve of MP50607-1, NDUFS5 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66053-2-PBS. Detection antibody: 66053-1-PBS. Standard:Ag7663. Range: 3.125-100 ng/mL.

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Product Information

66053-1-PBS targets NDUFS5 as part of a matched antibody pair:

MP50607-1: 66053-2-PBS capture and 66053-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: NDUFS5 fusion protein Ag7663
• Full Name: NADH dehydrogenase (ubiquinone) Fe-S protein 5, 15kDa (NADH-coenzyme Q reductase)
• Calculated Molecular Weight: 13 kDa
• Observed Molecular Weight: 15 kDa
• GenBank Accession Number: BC001884
• Gene Symbol: NDUFS5
• Gene ID (NCBI): 4725
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

NDUFS5 gene is a member of the NADH dehydrogenase (ubiquinone) iron-sulfur protein family. The encoded protein is a subunit of the NADH:ubiquinone oxidoreductase (complex I), the first enzyme complex in the electron transport chain located in the inner mitochondrial membrane. The NDUFS5 mRNA is expressed ubiquitously in human tissues, with a relative higher expression in human heart, skeletal muscle, liver, kidney and fetal heart (PMID: 10070614). The NDUFS5 (15 kDa) subunit located at mitochondrion and contains two C-X9-C motifs that are predicted to form a helix-coil-helix structure, permitting the formation of intramolecular disulfide bonds (PMID: 21310150). Proteinteach's NDUFS5 antibody 66053-1-Ig is capable of detecting the 15 kDa protein in Western blotting.