NDUFS1 Monoclonal antibody

NDUFS1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

3H5E8

Cat no : 68253-1-Ig

Synonyms

EC:7.1.1.2, Complex I-75kD, Complex I 75kD, CI-75kD, CI 75Kd

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Validation Data Gallery

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  WB analysis using 68253-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68253-1-Ig (NDUFS1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Lamin B1 Monoclonal antibody (66095-1-Ig) as loading control.

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  WB analysis of HeLa using 68253-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 68253-1-Ig (NDUFS1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse heart using 68253-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 68253-1-Ig (NDUFS1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 68253-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 68253-1-Ig (NDUFS1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 68253-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using NDUFS1 antibody (68253-1-Ig, Clone: 3H5E8 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

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  IF Staining of HepG2 using 68253-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using NDUFS1 antibody (68253-1-Ig, Clone: 3H5E8 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: pig brain tissue, HeLa cells, rabbit brain tissue, rat brain tissue, mouse brain tissue, pig heart tissue, rat heart tissue, mouse heart tissue
• Positive IHC detected in: mouse heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

68253-1-Ig targets NDUFS1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

• Tested Reactivity: human, mouse, rat, pig, rabbit
• Cited Reactivity: rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: NDUFS1 fusion protein Ag3135
• Full Name: NADH dehydrogenase (ubiquinone) Fe-S protein 1, 75kDa (NADH-coenzyme Q reductase)
• Calculated Molecular Weight: 727 aa, 79 kDa
• Observed Molecular Weight: 68-81 kDa
• GenBank Accession Number: BC030833
• Gene Symbol: NDUFS1
• Gene ID (NCBI): 4719
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The multisubunit NADH:ubiquinone oxidoreductase (75 kDa subunit, mitochondrial)(NDUFS1) is the first enzyme complex in the electron transport chain of mitochondria. It is also named as Complex I-75kD. By use of chaotropic agents, complex I can be fragmented into 3 different fractions: a flavoprotein fraction, an iron-sulfur protein (IP) fraction, and a hydrophobic protein (HP) fraction. NDUFS1 is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. NDUFS1 has some isoforms with the molecular mass of 68-81 kDa.

Publications

Species	Application	Title
rat	IF	Arch Oral Biol; Correlation of disulfidptosis and periodontitis: New insights and clinical significance; Authors - Yixin Fan