NDUFB9 Polyclonal antibody

NDUFB9 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 15572-1-AP

Synonyms

LYRM3, LYR motif-containing protein 3, Complex I-B22, CI-B22, CI B22

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Validation Data Gallery

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  WB analysis using 15572-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15572-1-AP (NDUFB9 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 15572-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15572-1-AP (NDUFB9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 15572-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15572-1-AP (NDUFB9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 15572-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15572-1-AP (NDUFB9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, mouse pancreas tissue, rat pancreas tissue
• Positive IHC detected in: human liver tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

15572-1-AP targets NDUFB9 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NDUFB9 fusion protein Ag7916
• Full Name: NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 9, 22kDa
• Calculated Molecular Weight: 22 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: BC007672
• Gene Symbol: NDUFB9
• Gene ID (NCBI): 4715
• RRID: AB_2267110
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Nat Commun; ZHX2 emerges as a negative regulator of mitochondrial oxidative phosphorylation during acute liver injury; Authors - Yankun Zhang
mouse	WB	Circulation; Nuclear Receptor NR1D1 Regulates Abdominal Aortic Aneurysm Development by Targeting the Mitochondrial Tricarboxylic Acid Cycle Enzyme Aconitase-2; Authors - Ling-Yue Sun