NDUFB8 Recombinant antibody

NDUFB8 Recombinant Antibody for WB, IF, FC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

230510H1

Cat no : 83216-4-RR

Synonyms

ASHI, CI ASHI, Complex I ASHI, NDUFB8



Tested Applications

Positive WB detected inHepG2 cells, A549 cells, HeLa cells, Jurkat cells, mouse liver tissue, mouse brain tissue, rat brain tissue
Positive IF detected inHepG2 cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunofluorescence (IF)IF : 1:125-1:500
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83216-4-RR is NDUFB8 Fusion Protein expressed in E. coli.

Tested Reactivity Human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen NDUFB8 fusion protein Ag6495
Full Name NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 8, 19kDa
Calculated Molecular Weight 22 kDa
Observed Molecular Weight 19-22 kDa
GenBank Accession NumberBC000466
Gene Symbol NDUFB8
Gene ID (NCBI) 4714
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for NDUFB8 antibody 83216-4-RRDownload protocol
IF protocol for NDUFB8 antibody 83216-4-RRDownload protocol
FC protocol for NDUFB8 antibody 83216-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols