WB analysis using 83216-4-RR (same clone as 83216-4-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 83216-4-RR (NDUFB8 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83216-4-PBS in a different storage buffer formulation.
Various lysates were subjected to SDS PAGE followed by western blot with 83216-4-RR (NDUFB8 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83216-4-PBS in a different storage buffer formulation.
IHC staining of human intrahepatic cholangiocarcinoma using 83216-4-RR (same clone as 83216-4-PBS)
Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83216-4-RR (NDUFB8 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83216-4-PBS in a different storage buffer formulation.
Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83216-4-RR (NDUFB8 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83216-4-PBS in a different storage buffer formulation.
IF Staining of HepG2 using 83216-4-RR (same clone as 83216-4-PBS)
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NDUFB8 antibody (83216-4-RR, Clone: 230510H1 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83216-4-PBS in a different storage buffer formulation.
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NDUFB8 antibody (83216-4-RR, Clone: 230510H1 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83216-4-PBS in a different storage buffer formulation.
FC experiment of HeLa using 83216-4-RR (same clone as 83216-4-PBS)
1x10^6 HeLa cells were intracellularly stained with 0.25 ug NDUFB8 Recombinant antibody (83216-4-RR, Clone:230510H1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83216-4-PBS in a different storage buffer formulation.
1x10^6 HeLa cells were intracellularly stained with 0.25 ug NDUFB8 Recombinant antibody (83216-4-RR, Clone:230510H1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83216-4-PBS in a different storage buffer formulation.
Affinity and Kinetic Characterization of 83216-4-RR
Biolayer interferometry (BLl) kinetic assays of 83216-4-RR against Human NDUFB8 were performed. The affinity constant is 5.00 nM.
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
WB analysis using 83216-4-RR (same clone as 83216-4-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 83216-4-RR (NDUFB8 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83216-4-PBS in a different storage buffer formulation.
IHC Figures
IHC staining of human intrahepatic cholangiocarcinoma using 83216-4-RR (same clone as 83216-4-PBS)
Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83216-4-RR (NDUFB8 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83216-4-PBS in a different storage buffer formulation.
IF/ICC Figures
IF Staining of HepG2 using 83216-4-RR (same clone as 83216-4-PBS)
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NDUFB8 antibody (83216-4-RR, Clone: 230510H1 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83216-4-PBS in a different storage buffer formulation.
FC (INTRA) Figures
FC experiment of HeLa using 83216-4-RR (same clone as 83216-4-PBS)
1x10^6 HeLa cells were intracellularly stained with 0.25 ug NDUFB8 Recombinant antibody (83216-4-RR, Clone:230510H1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83216-4-PBS in a different storage buffer formulation.
AFFINITY Figures
Affinity and Kinetic Characterization of 83216-4-RR
Biolayer interferometry (BLl) kinetic assays of 83216-4-RR against Human NDUFB8 were performed. The affinity constant is 5.00 nM.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
At Proteintech, we pride ourselves on our antibody quality, customer service and transparency. As such, we are comparing our antibodies with other vendors, enabling easy identification and comparisons of key data to help you choose the suitable antibody for your needs.
We have selected the top cited antibodies from these vendors for you to compare.
Proteintech
NDUFB8 Recombinant antibody
Catalog Number
83216-4-PBS
Citations
-
Dilutions
Applications
WB, IHC, IF/ICC, FC (Intra), ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
