NDUFB8 Recombinant antibody, PBS Only (Capture/Detector)

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  Sandwich ELISA standard curve of MP00204-2

  Sandwich ELISA standard curve of MP00204-2, Human NDUFB8 Recombinant Matched Antibody Pair - PBS only. 83216-6-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag6495. 83216-1-PBS was HRP conjugated as the detection antibody. Range: 3.13-200 ng/mL.

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  Sample test of MP00204-2

  The mean NDUFB8 concentration was determined to be 80.40 ng/mL in HeLa cell extract based on a 1.5 mg/mL extract load.

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  Cytometric bead array standard curve of MP00204-1

  Cytometric bead array standard curve of MP00204-1, NDUFB8 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83216-1-PBS. Detection antibody: 83216-2-PBS. Standard: Ag6495. Range: 2.5-20 ng/mL.

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  Affinity and Kinetic Characterization of 83216-1-PBS

  Biolayer interferometry (BLl) kinetic assay of 83216-1-PBS against Human NDUFB8 was performed. The affinity constant is below 1 pM.

NDUFB8 {ptg:Brand} Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 83216-1-PBS

Clone No.230510E10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

Complex I-ASHI, CI-ASHI, CIASHI, CI ASHI, ASHI

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Product Information

83216-1-PBS targets NDUFB8 as part of a matched antibody pair:

MP00204-1: 83216-1-PBS capture and 83216-2-PBS detection (validated in Cytometric bead array)

MP00204-2: 83216-6-PBS capture and 83216-1-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: NDUFB8 fusion protein Ag6495
• Full Name: NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 8, 19kDa
• Calculated Molecular Weight: 22 kDa
• GenBank Accession Number: BC000466
• Gene Symbol: NDUFB8
• Gene ID (NCBI): 4714
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O95169
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.