NDUFB7 Polyclonal antibody

NDUFB7 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

9

Cat no : 14912-1-AP

Synonyms

B18, Cell adhesion protein SQM1, CI B18, Complex I B18, NDUFB7

---

Validation Data Gallery

• 

  WB analysis of mouse ovary using 14912-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 14912-1-AP (NDUFB7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 14912-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 14912-1-AP (NDUFB7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 14912-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14912-1-AP (NDUFB7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 14912-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 14912-1-AP (NDUFB7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 14912-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 14912-1-AP (NDUFB7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse ovary using 14912-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 14912-1-AP (NDUFB7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 14912-1-AP

  IP result of anti-NDUFB7 (IP:14912-1-AP, 3ug; Detection:14912-1-AP 1:500) with mouse brain tissue lysate 2640ug.

• 

  IHC staining of human brain using 14912-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 14912-1-AP (NDUFB7 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 14912-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 14912-1-AP (NDUFB7 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human liver using 14912-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 14912-1-AP (NDUFB7 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver using 14912-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 14912-1-AP (NDUFB7 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human spleen using 14912-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14912-1-AP (NDUFB7 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 14912-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14912-1-AP (NDUFB7 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human heart using 14912-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 14912-1-AP (NDUFB7 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 14912-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 14912-1-AP (NDUFB7 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 14912-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 14912-1-AP (NDUFB7 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 14912-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 14912-1-AP (NDUFB7 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung using 14912-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 14912-1-AP (NDUFB7 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung using 14912-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 14912-1-AP (NDUFB7 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human cervical cancer using 14912-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14912-1-AP (NDUFB7 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human cervical cancer using 14912-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14912-1-AP (NDUFB7 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 14912-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14912-1-AP (NDUFB7 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 14912-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14912-1-AP (NDUFB7 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human placenta using 14912-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 14912-1-AP (NDUFB7 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human placenta using 14912-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 14912-1-AP (NDUFB7 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human testis using 14912-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14912-1-AP (NDUFB7 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 14912-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14912-1-AP (NDUFB7 antibody) at dilution of 1:100 (under 40x lens).

---

Tested Applications

• Positive WB detected in: mouse ovary tissue, MCF-7 cells, mouse brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human brain tissue, human cervical cancer tissue, human heart tissue, human kidney tissue, human liver tissue, human lung tissue, human placenta tissue, human spleen tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 9 publications below

Product Information

14912-1-AP targets NDUFB7 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NDUFB7 fusion protein Ag6709
• Full Name: NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 7, 18kDa
• Calculated Molecular Weight: 16 kDa
• Observed Molecular Weight: 18-22 kDa
• GenBank Accession Number: BC002595
• Gene Symbol: NDUFB7
• Gene ID (NCBI): 4713
• RRID: AB_2235903
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NDUFB7(NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7) is also named as CI-B18 and belongs to the complex I NDUFB7 subunit family. It is an accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), which couples the oxidation of NADH to the reduction of ubiquinone and the translocation of protons across the inner membrane. NDUFB7 protein lacks a mitochondrial targeting signal and transmembrane domains. However, it has a Cx(9)C motif that includes an intermembrane space targeting signal.(PMID:21310150).

Publications

Species	Application	Title
mouse	WB	Mol Metab; A disease-associated Aifm1 variant induces severe myopathy in knockin mice.; Authors - Lena Wischhof
mouse	WB	Ecotoxicol Environ Saf; Proteomic analysis reveals that cigarette smoke exposure diminishes ovarian reserve in mice by disrupting the CREB1-mediated ovarian granulosa cell proliferation-apoptosis balance; Authors - Mengting Xu
mouse	WB	Cell Death Dis; Loss of apoptosis-inducing factor critically affects MIA40 function.; Authors - K Meyer
human,mouse	WB	iScience; Mutational burden of XPNPEP3 leads to defects in mitochondrial complex I and cilia in NPHPL1; Authors - Lingxiao Tong
human	WB	J Biol Chem; A Point Mutation in the Exon Junction Complex Factor Y14 Disrupts Its Function in mRNA Cap Binding and Translation Enhancement.; Authors - Tzu-Wei Chuang
	WB	Mol Med; Altered redox mitochondrial biology in the neurodegenerative disorder fragile X-tremor/ataxia syndrome: use of antioxidants in precision medicine.; Authors - Gyu Song