NDUFAF2 Polyclonal antibody

NDUFAF2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 13891-1-AP

Synonyms

B17.2 like, B17.2L, mimitin, Mimitin, mitochondrial, MMTN, NDUFA12 like protein, NDUFA12L, NDUFAF2

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Validation Data Gallery

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  WB analysis of HepG2 using 13891-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13891-1-AP (NDUFAF2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of SH-SY5Y using 13891-1-AP

  IP result of anti-NDUFAF2 (IP:13891-1-AP, 4ug; Detection:13891-1-AP 1:500) with SH-SY5Y cells lysate 1600ug.

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  IHC staining of mouse skeletal muscle using 13891-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 13891-1-AP (NDUFAF2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skeletal muscle using 13891-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 13891-1-AP (NDUFAF2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HepG2 cells
• Positive IP detected in: SH-SY5Y cells
• Positive IHC detected in: mouse skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IP: See 1 publications below

Product Information

13891-1-AP targets NDUFAF2 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NDUFAF2 fusion protein Ag4853
• Full Name: NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, assembly factor 2
• Calculated Molecular Weight: 169 aa, 20 kDa
• Observed Molecular Weight: 25 kDa
• GenBank Accession Number: BC033965
• Gene Symbol: NDUFAF2
• Gene ID (NCBI): 91942
• RRID: AB_10597105
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Mimitin, also named as MMTN, B17.2L, NDUFAF2 and NDUFA12L, is a small mitochondrial protein upregulated by proinflammatory cytokines at the transcriptional and protein levels. It is reported as a possible chaperone for assembly of mitochondrial complex I. The MW of Mimitin is 17-25 kDa.

Publications

Species	Application	Title
human	WB	EMBO J; Respiratory supercomplexes act as a platform for complex III-mediated maturation of human mitochondrial complexes I and IV.; Authors - Margherita Protasoni
human	WB	J Med Genet; Respiratory chain complex I deficiency due to NDUFA12 mutations as a new cause of Leigh syndrome.; Authors - Ostergaard Elsebet E
human	IP	Redox Rep; Ndufaf2, a protein in mitochondrial complex I, interacts in vivo with methionine sulfoxide reductases; Authors - Sujin Park