NDUFA3 Polyclonal antibody

NDUFA3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 17257-1-AP

Synonyms

NADH-ubiquinone oxidoreductase B9 subunit, Complex I-B9, CI-B9, CIB9, CI B9

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Validation Data Gallery

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  WB analysis of human brain using 17257-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 17257-1-AP (NDUFA3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 17257-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17257-1-AP (NDUFA3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 17257-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17257-1-AP (NDUFA3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0).

• 

  IHC staining of human stomach using 17257-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 17257-1-AP (NDUFA3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 17257-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17257-1-AP (NDUFA3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0).

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Tested Applications

• Positive WB detected in: human brain tissue, HeLa cells
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below

Product Information

17257-1-AP targets NDUFA3 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NDUFA3 fusion protein Ag10912
• Full Name: NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 3, 9kDa
• Calculated Molecular Weight: 65aa,7 kDa; 84aa,9 kDa
• Observed Molecular Weight: 9 kDa
• GenBank Accession Number: BC011021
• Gene Symbol: NDUFA3
• Gene ID (NCBI): 4696
• RRID: AB_2150631
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	iScience; Mutational burden of XPNPEP3 leads to defects in mitochondrial complex I and cilia in NPHPL1; Authors - Lingxiao Tong
human	WB	J Biol Chem; Complex I mutations synergize to worsen the phenotypic expression of Leber's hereditary optic neuropathy.; Authors - Yanchun Ji
human	WB	Invest Ophthalmol Vis Sci; Assocation Between Leber's Hereditary Optic Neuropathy and MT-ND1 3460G>A Mutation-Induced Alterations in Mitochondrial Function, Apoptosis, and Mitophagy.; Authors - Juanjuan Zhang