NDEL1 Monoclonal antibody

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  WB analysis using 68954-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68954-1-Ig (NDEL1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

NDEL1 Monoclonal Antibody for WB, ELISA

Cat No. 68954-1-Ig

Clone No.1B3E9

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig

Applications

WB, ELISA

Synonyms

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Tested Applications

• Positive WB detected in: LNCaP cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, pig brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68954-1-Ig targets NDEL1 in WB, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: NDEL1 fusion protein Ag11197
• Full Name: nudE nuclear distribution gene E homolog (A. nidulans)-like 1
• Calculated Molecular Weight: 345 aa, 38 kDa, 41 kDa
• Observed Molecular Weight: 38-42 kDa
• GenBank Accession Number: BC026101
• Gene Symbol: NDEL1
• Gene ID (NCBI): 81565
• RRID: AB_3670456
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9GZM8
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Nuclear distribution factor E-homolog 1 (NDE1), NDE-like 1 (NDEL1), and Lissencephaly 1 (LIS1) together involve in essential neurodevelopmental processes, including neuronal precursor proliferation and differentiation, neurite outgrowth, and neuronal migration. All three bind directly to Disrupted in Schizophrenia 1 (DISC1), a scaffold protein critical to neuronal proliferation, integration, migration, and synaptic function within the developing and adult brain.[PMID: 18469341] Nuclear distribution element-like 1 (NDEL1) was firstly identified as a regulator of the cytoskeleton in microtubule and intermediate fliament dynamics and microtubule-based transport [PMID:21948775]. It is required for organization of the cellular microtubule array and microtubule anchoring at the centrosome, which is in part by targeting the microtubule severing protein KATNA1 to the centrosome[PMID:15728732].