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NDC80 Monoclonal antibody, PBS Only

NDC80 Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat, mouse

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2B7A8

Cat no : 66960-1-PBS

Synonyms

HEC, HEC1, HsHec1, hsNDC80, Kinetochore protein Hec1, KNTC2, NDC80, TID3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NDC80 Monoclonal antibody (66960-1-Ig)

    WB analysis using 66960-1-Ig (same clone as 66960-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66960-1-Ig (NDC80 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66960-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using NDC80 Monoclonal antibody (66960-1-Ig)

    WB analysis using 66960-1-Ig (same clone as 66960-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66960-1-Ig (NDC80 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66960-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HSC-T6 cells using NDC80 Monoclonal antibody (66960-1-Ig)

    WB analysis of HSC-T6 using 66960-1-Ig (same clone as 66960-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66960-1-Ig (NDC80 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66960-1-PBS in a different storage buffer formulation.

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66960-1-PBS targets NDC80 in WB, Indirect ELISA applications and shows reactivity with Human, rat, mouse samples.

Tested Reactivity Human, rat, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen NDC80 fusion protein Ag8065
Full Name NDC80 homolog, kinetochore complex component (S. cerevisiae)
Calculated Molecular Weight 74 kDa
Observed Molecular Weight 71-74 kDa
GenBank Accession NumberBC035617
Gene Symbol NDC80
Gene ID (NCBI) 10403
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.