MT-ND5 Polyclonal antibody

MT-ND5 Polyclonal Antibody for IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 31132-1-AP

Synonyms

ND5, ND 5, NADH5, MTND5, MT ND5

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Validation Data Gallery

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  IF Staining of HepG2 using 31132-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ND5 antibody (31132-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31132-1-AP targets MT-ND5 in IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: fusion protein
• Full Name: NADH dehydrogenase, subunit 5 (complex I)
• Calculated Molecular Weight: 67 kDa
• GenBank Accession Number: YP_003024036
• Gene Symbol: MT-ND5
• Gene ID (NCBI): 4540
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ND5 belongs to the complex I subunit 1 family. ND5 is a core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.