ND1 Polyclonal antibody

• Featured Product
• KD/KO Validated

ND1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (3)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

77

Reviews (3)

Cat no : 19703-1-AP

Synonyms

MT-ND1, NADH1, MTND1, MT ND1

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Validation Data Gallery

• 

  WB analysis using 19703-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19703-1-AP (ND1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 19703-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19703-1-AP (ND1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 19703-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 19703-1-AP (ND1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 19703-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 19703-1-AP (ND1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse brain tissue, mouse liver tissue, mouse heart tissue, mouse kidney tissue, mouse liver mitochondria
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 74 publications below
• IHC: See 4 publications below
• IF: See 1 publications below

Product Information

19703-1-AP targets ND1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat, pig, hamster
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: NADH dehydrogenase, subunit 1 (complex I)
• Calculated Molecular Weight: 36 kDa
• Observed Molecular Weight: 28-38 kDa
• GenBank Accession Number: NC_012920
• Gene Symbol: ND1
• Gene ID (NCBI): 4535
• RRID: AB_10637853
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ND1 belongs to the complex I subunit 1 family. ND1 is a core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

Publications

Species	Application	Title
human	IF	Cell; Single-Cell Transcriptomic Atlas of Primate Ovarian Aging.; Authors - Si Wang
mouse	WB	Cell; MicroRNA Directly Enhances Mitochondrial Translation during Muscle Differentiation.; Authors - Xiaorong Zhang ; KD Validated
mouse	IHC	Circulation; Extracellular Tuning of Mitochondrial Respiration Leads to Aortic Aneurysm.; Authors - Jorge Oller
human	WB	Mol Cell; Filamentous GLS1 promotes ROS-induced apoptosis upon glutamine deprivation via insufficient asparagine synthesis.; Authors - Bin Jiang
human	WB	Sci Adv; MCART1/SLC25A51 is required for mitochondrial NAD transport.; Authors - Nora Kory
mouse	WB	Nat Commun; Disuse-associated loss of the protease LONP1 in muscle impairs mitochondrial function and causes reduced skeletal muscle mass and strength.; Authors - Zhisheng Xu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Tania (Verified Customer) (03-05-2022)

a lot of background. difficult to say each band is the correct one.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: ES2 human cells

FH

Daniel (Verified Customer) (10-01-2019)

ND2 was detected in both liver (L) and heart (H) lysate from a wildtype mouse. No ND1 was detected in samples processed in parallel.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse liver (L), heart (H)

ND1 Antibody Western Blot, validation (1:1000 dilution) in Mouse liver (L), heart (H) (Cat no:19703-1-AP)

FH

Loukmane (Verified Customer) (08-10-2018)

It works very well with A549 and lung tissue, although with the former the background is higherThe image shows ND1 in whole A549 lysate

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Lung tissue and A549

ND1 Antibody Western Blot, validation (1:500 dilution) in Lung tissue and A549 (Cat no:19703-1-AP)

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