NCX1 Polyclonal antibody

NCX1 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse and More (1)

Applications

WB, IHC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

11

Cat no : 55075-1-AP

Synonyms

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Validation Data Gallery

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  WB analysis of mouse brain using 55075-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55075-1-AP (NCX1 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 55075-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 55075-1-AP (NCX1 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 55075-1-AP

  IP result of anti-NCX1 (IP:55075-1-AP, 4ug; Detection:55075-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 9 publications below
• IHC: See 1 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Product Information

55075-1-AP targets NCX1 in WB, IHC, IP, CoIP, ELISA applications and shows reactivity with human, rat, mouse samples.

• Tested Reactivity: human, rat, mouse
• Cited Reactivity: human, mouse, rat, chicken
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: solute carrier family 8 (sodium/calcium exchanger), member 1
• Calculated Molecular Weight: 109 kDa
• Observed Molecular Weight: 120 kDa
• GenBank Accession Number: NM_021097
• Gene Symbol: NCX1
• Gene ID (NCBI): 6546
• RRID: AB_2881262
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: 0.1M NaHCO3, 0.1M glycine, 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB,IHC	J Am Soc Nephrol; Gentamicin Inhibits Ca2+ Channel TRPV5 and Induces Calciuresis Independent of the Calcium-Sensing Receptor-Claudin-14 Pathway.; Authors - Wouter H van Megen
rat	WB	Front Immunol; Interleukin-6-Mediated-Ca2+ Handling Abnormalities Contributes to Atrial Fibrillation in Sterile Pericarditis Rats.; Authors - Jie Liao
mouse	IP	J Am Heart Assoc; Sodium-Glucose Cotransporter 2 Inhibitor Canagliflozin Antagonizes Salt-Sensitive Hypertension Through Modifying Transient Receptor Potential Channels 3 Mediated Vascular Calcium Handling.; Authors - Yu Zhao
chicken	WB	Chemosphere; NF-κB-mediated inflammation correlates with calcium overload under arsenic trioxide-induced myocardial damage in Gallus gallus.; Authors - Siwen Li
mouse	WB	Alcohol Clin Exp Res; Impact and related mechanisms of chronic moderate alcohol consumption on cardiac function in aldehyde dehydrogenase 2 heterozygous mice.; Authors - Qinfeng Hu
rat	CoIP	Front Physiol; PMID: 32009985; Authors - Raúl Sampieri