NCX1 Polyclonal antibody

NCX1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 28447-1-AP

Synonyms

CNC, DKFZp779F0871, FLJ37694, FLJ43417, NCX1, SLC8A1, Sodium/calcium exchanger 1

---

Validation Data Gallery

• 

  WB analysis using 28447-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28447-1-AP (NCX1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 28447-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 28447-1-AP (NCX1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 28447-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 28447-1-AP (NCX1 antibody) at dilution of 1:1600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: mouse heart tissue, rat heart tissue, rat kidney tissue
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:200-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

28447-1-AP targets NCX1 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NCX1 fusion protein Ag28952
• Full Name: solute carrier family 8 (sodium/calcium exchanger), member 1
• Calculated Molecular Weight: 109 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: NM_021097
• Gene Symbol: NCX1
• Gene ID (NCBI): 6546
• RRID: AB_2918167
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	J Neuroinflammation; Sexually dimorphic extracellular vesicle responses after chronic spinal cord injury are associated with neuroinflammation and neurodegeneration in the aged brain; Authors - Yun Li
mouse	WB	Acta Pharmacol Sin; Porcupine inhibitor CGX1321 alleviates heart failure with preserved ejection fraction in mice by blocking WNT signaling; Authors - Hao Wu