SRC3/NCOA3 Polyclonal antibody

• Featured Product
• KD/KO Validated

SRC3/NCOA3 Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF, IHC, ChIP, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 20032-1-AP

Synonyms

ACTR, AIB 1, AIB1, CBP interacting protein, KAT13B, NCoA 3, NCOA3, nuclear receptor coactivator 3, pCIP, RAC 3, RAC3, SRC 3, SRC3, SRC3/NCOA3, TNRC14, TNRC16, TRAM 1, TRAM1

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Validation Data Gallery

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  WB analysis of HeLa using 20032-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20032-1-AP (NCOA3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 20032-1-AP

  Immunofluorescent analysis of HepG2 cells, using NCOA3 antibody 20032-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: HeLa cells
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 2 publications below
• IHC: See 3 publications below
• ChIP: See 1 publications below

Product Information

20032-1-AP targets SRC3/NCOA3 in WB, IF, IHC, ChIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: nuclear receptor coactivator 3
• Calculated Molecular Weight: 155 kDa
• Observed Molecular Weight: 160 kDa
• GenBank Accession Number: NM_006534
• Gene Symbol: NCOA3
• Gene ID (NCBI): 8202
• RRID: AB_10666164
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NCOA3, also named as AIB1, SRC3 and TRAM1, belongs to the SRC/p160 nuclear receptor coactivator family. NCOA3 is a nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. NOCA3 plays a central role in creating a multisubunit coactivator complex, which probably acts via remodeling of chromatin. Involved in the coactivation of different nuclear receptors, such as for steroids (GR and ER), retinoids (RARs and RXRs), thyroid hormone (TRs), vitamin D3 (VDR) and prostanoids (PPARs). NCOA3 also involved in the coactivation of the NF-kappa-B pathway via its interaction with the NFKB1 subunit. The antibody can recognizes all the isoforms of NCOA3.

Publications

Species	Application	Title
human,mouse	ChIP	J Biol Chem; A potential target for liver cancer management, lysophosphatidic acid receptor 6 (LPAR6), is transcriptionally up-regulated by the NCOA3 coactivator; Authors - Xuan Zheng ; KD Validated
human	WB	J Biol Chem; A potential target for liver cancer management, lysophosphatidic acid receptor 6 (LPAR6), is transcriptionally up-regulated by the NCOA3 coactivator.; Authors - Xuan Zheng ; KD Validated
human	WB,IHC	Biomed Pharmacother; PMID: 32036219; Authors - Yu Chen ; KD Validated
human	IHC	Medicine (Baltimore); Pathological discrimination between luteinized thecoma associated with sclerosing peritonitis and thecoma; Authors - Jia Liu
human	IHC	Ann Diagn Pathol; Evaluation of the diagnostic utility of NCOA3, Maspin and VHL protein expression in pancreatic ductal adenocarcinoma: An immunohistochemical study; Authors - Noha Elkady