NCOA2 Polyclonal antibody

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  WB analysis using 30962-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30962-1-AP (NCOA2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  IF Staining of U2OS using 30962-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using NCOA2 antibody (30962-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of HepG2 using 30962-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NCOA2 antibody (30962-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

NCOA2 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 30962-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

NCoA 2, KAT13C, hTIF2, GRIP1, Class E basic helix-loop-helix protein 75

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Tested Applications

• Positive WB detected in: HEK-293T cells, HepG2 cells, Jurkat cells, LNCaP cells
• Positive IF/ICC detected in: HepG2 cells, U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below

Product Information

30962-1-AP targets NCOA2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NCOA2 fusion protein Ag33768
• Full Name: nuclear receptor coactivator 2
• Calculated Molecular Weight: 1464 aa, 159 kDa
• Observed Molecular Weight: 160-180 kDa
• GenBank Accession Number: BC114383
• Gene Symbol: NCOA2
• Gene ID (NCBI): 10499
• RRID: AB_3669796
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q15596
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
rat	IHC	Phytomedicine; Yiqi Huayu Jiedu formula inhibits JAK2/STAT3-mediated partial EMT in treating chronic atrophic gastritis; Authors - Weifeng Yu