NCKAP5 Polyclonal antibody

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  WB analysis using 31199-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31199-1-AP (NCKAP5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 31199-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31199-1-AP (NCKAP5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IF Staining of U2OS using 31199-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using NCKAP5 antibody (31199-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

NCKAP5 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 31199-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Synonyms

NAP5, Peripheral clock protein, Nck associated protein 5, NAP 5, ERIH2

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Tested Applications

• Positive WB detected in: mouse brain tissue, HepG2 cells
• Positive IF/ICC detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31199-1-AP targets NCKAP5 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NCKAP5 fusion protein Ag35092
• Full Name: Nck-associated protein 5
• Calculated Molecular Weight: 209 kDa
• Observed Molecular Weight: 250-260 kDa
• GenBank Accession Number: NM_207363
• Gene Symbol: NAP5
• Gene ID (NCBI): 344148
• RRID: AB_3669897
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: O14513
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NCKAP5 (Nck-associated protein 5), also known as NAP5. NCKAP5 is predicted to be located in cells, especially in nucleoli, Golgi apparatus, cell membrane and cytoplasm. It is widely expressed in many tissues, especially in lung and kidney, and in more than 20 other tissues. And the expression of NCKAP5 was enhanced in oligodendrocytes, oligodendrocyte precursor cells and astrocytes. It may interact with other protein, such as NPM1 and centromere protein complex, and regulate accurate chromosome segregation and distribution. NCKAP5 may be involved in the formation and development of tumors, especially in the process of tumor variation such as ovary and breast cancer. In addition, NCKAP5 may be a diagnostic biomarker in non-small cell lung cancer (NSCLC), which is related to the infiltration of activated B cells, regulatory T cells (Tregs), neutrophils and dendritic cells (PMID: 36628881). The molecular weight of NCKAP5 is 208 kDa, it also has some protein modifications.