NCK2 Polyclonal antibody

• Featured Product
• KD/KO Validated

NCK2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 10206-1-AP

Synonyms

Cytoplasmic protein NCK2, GRB4, Nck 2, NCK adaptor protein 2, NCK2, NCKbeta

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Validation Data Gallery

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  WB analysis of U-937 using 10206-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 10206-1-AP (NCK2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U-937 using 10206-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 10206-1-AP (NCK2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human placenta using 10206-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10206-1-AP (NCK2 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human placenta using 10206-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10206-1-AP (NCK2 Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: U-937 cells
• Positive IHC detected in: human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below

Product Information

10206-1-AP targets NCK2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NCK2 fusion protein Ag0262
• Full Name: NCK adaptor protein 2
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 43 kDa
• GenBank Accession Number: BC000103
• Gene Symbol: NCK2
• Gene ID (NCBI): 8440
• RRID: AB_2150571
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Lots of the protein-protein interactions that are essential for eukaryotic intracellular signal transduction are mediated by protein binding modules including SH2, SH3, and LIM domains. NCK is a SH3- and SH2-containing adaptor protein implicated in coordinating various signaling pathways, including those of growth factor receptors and cell adhesion receptors. NCK-2 is a ubiquitously expressed and NCK-related adaptor protein comprising primarily three N-terminal SH3 domains and one C-terminal SH2 domain. NCK-2 interacts with PINCH, a LIM-only protein implicated in integrin-linked kinase signaling, and recognizes several key components of growth factor kinase-signaling pathways including EGF receptors, PDGF receptor, and IRS-1. NCK-2 may thus function as an adaptor protein connecting the growth factor receptor-signaling pathways with the integrin-signaling pathways. NCK-2 also interacts with focal adhesion kinase (FAK), a cytoplasmic protein tyrosine kinase critically involved in the cellular control of motility.

Publications

Species	Application	Title
mouse	WB	Evid Based Complement Alternat Med; Icariin Alleviates Glucocorticoid-Induced Osteoporosis through EphB4/Ephrin-B2 Axis.; Authors - Mi Huang
human	WB	Biochem Pharmacol; KLF4 suppresses anticancer effects of brusatol via transcriptional upregulating NCK2 expression in melanoma; Authors - Xiaodong Li ; KD Validated