NCF4 Polyclonal antibody

NCF4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 14648-1-AP

Synonyms

P40PHOX, p40 phox, Neutrophil cytosol factor 4, NCF-4, NCF 4

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Validation Data Gallery

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  WB analysis of L02 using 14648-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 14648-1-AP (NCF4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 14648-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 14648-1-AP (NCF4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 14648-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 14648-1-AP (NCF4 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 14648-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 14648-1-AP (NCF4 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of U2OS using 14648-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using NCF4 antibody (14648-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Tested Applications

• Positive WB detected in: L02 cells
• Positive IHC detected in: human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

14648-1-AP targets NCF4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NCF4 fusion protein Ag6241
• Full Name: neutrophil cytosolic factor 4, 40kDa
• Calculated Molecular Weight: 39 kDa
• Observed Molecular Weight: 41 kDa
• GenBank Accession Number: BC002798
• Gene Symbol: NCF4
• Gene ID (NCBI): 4689
• RRID: AB_2251237
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NCF4, also named as p40phox, was the last NADPH oxidase subunit to be discovered, and although there is evidence of its both positive and negative effects on oxidase function. Catalog#14648-1-AP is a rabbit polyclonal antibody raised against the full-length of human NCF4.The MW of this protein is 40 kDa, and this antibody specially recognises the 40 kDa protein.

Publications

Species	Application	Title
rat		BMC Pulm Med; Simvastatin attenuates silica-induced pulmonary inflammation and fibrosis in rats via the AMPK-NOX pathway; Authors - Cunxiang Bo
rat	IF	CNS Neurosci Ther; Neutrophil migration participates in the side effect of recombinant human tissue plasminogen activator; Authors - Yuyou Huang