NCAPD3 Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

NCAPD3 Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 16828-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of HepG2 using 16828-1-AP (same clone as 16828-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16828-1-AP (NCAPD3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16828-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 16828-1-AP (same clone as 16828-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 16828-1-AP (NCAPD3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16828-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 16828-1-AP (same clone as 16828-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 16828-1-AP (NCAPD3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16828-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 16828-1-AP (same clone as 16828-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 16828-1-AP (NCAPD3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16828-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HeLa using 16828-1-AP (same clone as 16828-1-PBS)

  IP result of anti-NCAPD3 (IP:16828-1-AP, 4ug; Detection:16828-1-AP 1:500) with HeLa cells lysate 600ug. This data was developed using the same antibody clone with 16828-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 16828-1-AP (same clone as 16828-1-PBS)

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 16828-1-AP (NCAPD3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16828-1-PBS in a different storage buffer formulation.

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Product Information

16828-1-PBS targets NCAPD3 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NCAPD3 fusion protein Ag10429
• Full Name: non-SMC condensin II complex, subunit D3
• Calculated Molecular Weight: 1498 aa, 169 kDa
• Observed Molecular Weight: 165 kDa
• GenBank Accession Number: BC011408
• Gene Symbol: NCAPD3
• Gene ID (NCBI): 23310
• RRID: AB_2282528
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Condensin complexes I and II play essential roles in mitotic chromosome assembly and segregation. Both condensins contain 2 invariant structural maintenance of chromosome (SMC) subunits, SMC2 and SMC4 , but they contain different sets of non-SMC subunits. NCAPD3 is 1 of 3 non-SMC subunits that define condensin II ([PMID: 14532007).