NCAM2 Polyclonal antibody

NCAM2 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 13850-1-AP

Synonyms

NCAM21, NCAM-2, NCAM 2, N CAM 2

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Validation Data Gallery

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  WB analysis using 13850-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13850-1-AP (NCAM2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 13850-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13850-1-AP (NCAM2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 13850-1-AP

  IP result of anti-NCAM2 (IP:13850-1-AP, 4ug; Detection:13850-1-AP 1:5000) with mouse brain tissue lysate 2100 ug.

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  IHC staining of mouse brain using 13850-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13850-1-AP (NCAM2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 13850-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13850-1-AP (NCAM2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 13850-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13850-1-AP (NCAM2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 13850-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13850-1-AP (NCAM2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse brain tissue, human brain tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: mouse brain tissue, human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 2 publications below

Product Information

13850-1-AP targets NCAM2 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NCAM2 fusion protein Ag4821
• Full Name: neural cell adhesion molecule 2
• Calculated Molecular Weight: 93 kDa
• Observed Molecular Weight: 100-125 kDa
• GenBank Accession Number: BC052946
• Gene Symbol: NCAM2
• Gene ID (NCBI): 4685
• RRID: AB_2237029
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The mammalian NCAM family has two members, NCAM1 and NCAM2 (PMID: 9729879; 20439718). Neural cell adhesion molecule 2 (NCAM2, also known as NCAM21) is a single-pass type I membrane protein that is highly expressed in the brain, which may play important roles in selective fasciculation and zone-to-zone projection of the primary olfactory axons or in neurodegenerative diseases such as Alzheimer's disease (PMID: 26611261). NCAM2 has two isoforms: NCAM2.1 bearing transmembrane and cytoplasmic domains and the shorter NCAM2.2 isoform, which is GPI-anchored (PMID: 9334170; 9221781). The molecular weight of NCAM2 is around 125 kDa and 93 kDa (PMID: 9334170; 3171685).

Publications

Species	Application	Title
mouse	IHC	Science; Breast cancer exploits neural signaling pathways for bone-to-meninges metastasis; Authors - Andrew E Whiteley
		J Comp Neurol; Illuminating the terminal nerve: Uncovering the link between GnRH-1 neuron and olfactory development; Authors - Enrico Amato
mouse	IHC	bioRxiv; Illuminating the Terminal Nerve: Uncovering the Link between GnRH-1 and Olfactory Development; Authors - Enrico Amato