WB result of NCAM1/CD56 antibody (82715-1-RR; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NCAM1/CD56 transfected U-251 cells.
WB result of NCAM1/CD56 antibody (82715-1-RR; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NCAM1/CD56 transfected U-251 cells.
WB analysis using 82715-1-RR
Various lysates were subjected to SDS PAGE followed by western blot with 82715-1-RR (NCAM1/CD56 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 82715-1-RR (NCAM1/CD56 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of SH-SY5Y using 82715-1-RR
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 82715-1-RR (NCAM1/CD56 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 82715-1-RR (NCAM1/CD56 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of U-251 using 82715-1-RR
U-251 cells were subjected to SDS PAGE followed by western blot with 82715-1-RR (NCAM1/CD56 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
U-251 cells were subjected to SDS PAGE followed by western blot with 82715-1-RR (NCAM1/CD56 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
IHC staining of mouse brain using 82715-1-RR
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82715-1-RR (NCAM1/CD56 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82715-1-RR (NCAM1/CD56 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of SH-SY5Y using 82715-1-RR
Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using NCAM1/CD56 antibody (82715-1-RR, Clone: 6N11 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using NCAM1/CD56 antibody (82715-1-RR, Clone: 6N11 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
Affinity and Kinetic Characterization of 82715-1-RR
Biolayer interferometry (BLl) kinetic assays of 82715-1-RR against Human NCAM1 were performed. The affinity constant is 0.23 nM.
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mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
SH-SY5Y cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:2000-1:10000
Immunohistochemistry (IHC)
IHC : 1:200-1:800
Immunofluorescence (IF)/ICC
IF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
Neural cell adhesion molecule 1 (NCAM1, also known as CD56) is a cell adhesion glycoprotein of the immunoglobulin (Ig) superfamily. It is a multifunction protein involved in synaptic plasticity, neurodevelopment, and neurogenesis. NCAM1 is expressed on human neurons, glial cells, skeletal muscle cells, NK cells and a subset of T cells, and the expression is observed in a wide variety of human tumors, including myeloma, myeloid leukemia, neuroendocrine tumors, Wilms' tumor, neuroblastoma, and NK/T cell lymphomas. Three major isoforms of NCAM1, with molecular masses of 120, 140, and 180 kDa, are generated by alternative splicing of mRNA (PMID: 9696812). The glycosylphosphatidylinositol (GPI)-anchored NCAM120 and the transmembrane NCAM140 and NCAM180 consist of five Ig-like domains and two fibronection-type III repeats (FNIII). All three forms can be posttranslationally modified by addition of polysialic acid (PSA) (PMID: 14976519). Several other isofroms have also been described (PMID: 1856291).
Crosstalk between cancer-associated fibroblasts and non-neuroendocrine tumor cells in small cell lung cancer involves in glycolysis and antigen-presenting features
WB result of NCAM1/CD56 antibody (82715-1-RR; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NCAM1/CD56 transfected U-251 cells.
WB analysis using 82715-1-RR
Various lysates were subjected to SDS PAGE followed by western blot with 82715-1-RR (NCAM1/CD56 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of SH-SY5Y using 82715-1-RR
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 82715-1-RR (NCAM1/CD56 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of U-251 using 82715-1-RR
U-251 cells were subjected to SDS PAGE followed by western blot with 82715-1-RR (NCAM1/CD56 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of mouse brain using 82715-1-RR
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82715-1-RR (NCAM1/CD56 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 82715-1-RR
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82715-1-RR (NCAM1/CD56 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF/ICC Figures
IF Staining of SH-SY5Y using 82715-1-RR
Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using NCAM1/CD56 antibody (82715-1-RR, Clone: 6N11 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
AFFINITY Figures
Affinity and Kinetic Characterization of 82715-1-RR
Biolayer interferometry (BLl) kinetic assays of 82715-1-RR against Human NCAM1 were performed. The affinity constant is 0.23 nM.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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