NCAM1/CD56 Recombinant antibody

• Featured Product
• KD/KO Validated

NCAM1/CD56 Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Pig, Mouse, Rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

6N11

Cat no : 82715-1-RR

Synonyms

CD56, NCAM1, 6N11, NCAM 1, NCAM

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Validation Data Gallery

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  WB analysis of U-251 using 82715-1-RR

  WB result of NCAM1/CD56 antibody (82715-1-RR; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NCAM1/CD56 transfected U-251 cells.

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  WB analysis using 82715-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82715-1-RR (NCAM1/CD56 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 82715-1-RR

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 82715-1-RR (NCAM1/CD56 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of U-251 using 82715-1-RR

  U-251 cells were subjected to SDS PAGE followed by western blot with 82715-1-RR (NCAM1/CD56 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 82715-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82715-1-RR (NCAM1/CD56 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 82715-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82715-1-RR (NCAM1/CD56 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of SH-SY5Y using 82715-1-RR

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using NCAM1/CD56 antibody (82715-1-RR, Clone: 6N11 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  Affinity and Kinetic Characterization of 82715-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82715-1-RR against Human NCAM1 were performed. The affinity constant is 0.23 nM.

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Tested Applications

• Positive WB detected in: pig brain tissue, SH-SY5Y cells, U-251 cells, Neuro-2a cells, C6 cells, mouse brain tissue, rat brain tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82715-1-RR targets NCAM1/CD56 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Pig, Mouse, Rat samples.

• Tested Reactivity: Human, Pig, Mouse, Rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: NCAM1/CD56 fusion protein Ag5528
• Full Name: neural cell adhesion molecule 1
• Calculated Molecular Weight: 95 kDa
• Observed Molecular Weight: 120 kDa, 140 kDa, 180 kDa
• GenBank Accession Number: BC047244
• Gene Symbol: NCAM1
• Gene ID (NCBI): 4684
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Neural cell adhesion molecule 1 (NCAM1, also known as CD56) is a cell adhesion glycoprotein of the immunoglobulin (Ig) superfamily. It is a multifunction protein involved in synaptic plasticity, neurodevelopment, and neurogenesis. NCAM1 is expressed on human neurons, glial cells, skeletal muscle cells, NK cells and a subset of T cells, and the expression is observed in a wide variety of human tumors, including myeloma, myeloid leukemia, neuroendocrine tumors, Wilms' tumor, neuroblastoma, and NK/T cell lymphomas. Three major isoforms of NCAM1, with molecular masses of 120, 140, and 180 kDa, are generated by alternative splicing of mRNA (PMID: 9696812). The glycosylphosphatidylinositol (GPI)-anchored NCAM120 and the transmembrane NCAM140 and NCAM180 consist of five Ig-like domains and two fibronection-type III repeats (FNIII). All three forms can be posttranslationally modified by addition of polysialic acid (PSA) (PMID: 14976519). Several other isofroms have also been described (PMID: 1856291).