• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

NBN / NBS1 Recombinant antibody, PBS Only

NBN / NBS1 Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1C16

Cat no : 82884-1-PBS

Synonyms

AT V1, AT V2, ATV, FLJ10155, NBN, NBS, NBS1, nibrin, P95

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using NBN / NBS1 Recombinant antibody (82884-1-RR)

    WB analysis using 82884-1-RR (same clone as 82884-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82884-1-RR (NBN / NBS1 antibody) at dilution of 1:5100 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82884-1-PBS in a different storage buffer formulation.

Product Information

82884-1-PBS targets NBN / NBS1 in WB, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide
Full Name nibrin
Calculated Molecular Weight 85 kDa
Observed Molecular Weight 90-95 kDa
GenBank Accession NumberBC136803
Gene Symbol NBN
Gene ID (NCBI) 4683
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

NBN, also named as NBS, NBS1, and P95, is a component of the MRE11/RAD50/NBN (MRN complex) which plays a critical role in the cellular response to DNA damage and the maintenance of chromosome integrity. The complex is involved in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity, cell cycle checkpoint control, and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. NBN modulates the DNA damage signal sensing by recruiting PI3/PI4-kinase family members ATM, ATR, and probably DNA-PKcs to the DNA damage sites and activating their functions. NBN also functions in telomere length maintenance by generating the 3' overhang which serves as a primer for telomerase-dependent telomere elongation. NBN is a major player in the control of intra-S-phase checkpoints and there is some evidence that NBN is involved in G1 and G2 checkpoints. Defects in NBN are the cause of Nijmegen breakage syndrome (NBS). Defects in NBN are a cause of genetic susceptibility to breast cancer (BC). Defects in NBN may be associated with aplastic anemia. Defects in NBN might play a role in the pathogenesis of childhood acute lymphoblastic leukemia (ALL). The antibody is specific to NBN. The full-length NBN protein, with an apparent molecular weight of 95 kDa and the two protein fragments of 26 and 70 kDa arising from the c.657_661del5 (p.K219fsX19) mutation, and the 80 kDa protein found in patient RR with the mutation c.742_743insGG leading to excision of exons 6 and 7 from the NBN mRNA are shown. (PMID: 26265251) The predicted molecular weight of NBN protein (p95) is 85kDa, and actually detection result is about 95kDa(PMID: 23762398).