NBL1 Polyclonal antibody

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  WB analysis of mouse colon using 12069-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 12069-1-AP (NBL1 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

NBL1 Polyclonal Antibody for WB, ELISA

Cat No. 12069-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

D1S1733E, DAN, DAN domain family member 1, DAND1, NB, NBL1, NO3, Protein N03, Zinc finger protein DAN

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Tested Applications

• Positive WB detected in: mouse colon tissue

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12069-1-AP targets NBL1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NBL1 fusion protein Ag2706
• Full Name: neuroblastoma, suppression of tumorigenicity 1
• Calculated Molecular Weight: 180 aa, 19 kDa
• Observed Molecular Weight: 19 kDa
• GenBank Accession Number: BC012037
• Gene Symbol: NBL1
• Gene ID (NCBI): 4681
• RRID: AB_10638604
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P41271
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NBL1 (neuroblastoma suppression of tumorigenicity 1), also named as DAN, is the founding member of the DAN subfamily. It was originally identified as a putative tumor suppressor gene in a transformed fibroblast rat model (PMID: 9516839), whereby it was significantly down-regulated in v-src-transformed rat fibroblasts. The MW of this protein is 19 kDa, and this antibody specially recognises the 19 kDa protein.