NAT6/FUS2 Polyclonal antibody

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  WB analysis using 15476-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15476-1-AP (NAT6/FUS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 15476-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using NAT6/FUS2 antibody (15476-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

NAT6/FUS2 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 15476-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

NAA80, NAT6, N-acetyltransferase 6, HsNAAA80, FUS2

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Tested Applications

• Positive WB detected in: HeLa cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 1 publications below

Product Information

15476-1-AP targets NAT6/FUS2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NAT6/FUS2 fusion protein Ag7780
• Full Name: N-acetyltransferase 6 (GCN5-related)
• Calculated Molecular Weight: 31 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC004483
• Gene Symbol: FUS2
• Gene ID (NCBI): 24142
• RRID: AB_3085463
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q93015
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The acetyltransferase NAT6 (also known as Fus2) is an enzyme that catalyzes the transfer of acetyl groups from acetyl-CoA to acrylamines and located mainly in the cytoplasm. NAT6 has two isoforms. The molecular weight of NAT6 was detected to be about 45kDa (PMID:32978259).

Publications

Species	Application	Title
human	WB,IF	FEBS J; NAT6 acetylates the N-terminus of different forms of actin.; Authors - Elsa Wiame ; KO Validated
human	WB	Brain Commun; NAA80 bi-allelic missense variants result in high-frequency hearing loss, muscle weakness and developmental delay.; Authors - Irena J J Muffels