NAT2 Polyclonal antibody, PBS Only

• 

  WB analysis using 11410-1-AP (same clone as 11410-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11410-1-AP (NAT2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11410-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human small intestine using 11410-1-AP (same clone as 11410-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 11410-1-AP (NAT2 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 11410-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 11410-1-AP (same clone as 11410-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11410-1-AP (NAT2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11410-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 11410-1-AP (same clone as 11410-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11410-1-AP (NAT2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11410-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 11410-1-AP (same clone as 11410-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11410-1-AP (NAT2 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 11410-1-PBS in a different storage buffer formulation.

• 

  FC experiment of MCF-7 using 11410-1-AP (same clone as 11410-1-PBS)

  1x10^6 MCF-7 cells were intracellularly stained with 0.8 ug NAT2 Polyclonal antibody (11410-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.8 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 11410-1-PBS in a different storage buffer formulation.

NAT2 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 11410-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

---

Product Information

11410-1-PBS targets NAT2 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NAT2 fusion protein Ag1953
• Full Name: N-acetyltransferase 2 (arylamine N-acetyltransferase)
• Calculated Molecular Weight: 33.5 kDa
• Observed Molecular Weight: 38 kDa
• GenBank Accession Number: BC015878
• Gene Symbol: NAT2
• Gene ID (NCBI): 10
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P11245
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

NAT2 (Arylamine N-acetyltransferase 2) is well-known for its role in the phase II metabolism of xenobiotics and drugs (PMID:36156098). NAT2 and its isozyme, arylamine N-acetyltransferase 1 (NAT1), play key roles in the detoxification of carcinogenic arylamines and xenobiotics by catalyzing acetyl-coenzyme A (CoA)-dependent biotransformation of arylamines to N-arylacetamides. Endogenous NAT2 expression is limited to the liver and small and large intestines, suggesting that NAT2 plays organ-specific roles (PMID:24467436).