NAT2 Polyclonal antibody

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  WB analysis using 11410-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11410-1-AP (NAT2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IHC staining of human small intestine using 11410-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 11410-1-AP (NAT2 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human breast cancer using 11410-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11410-1-AP (NAT2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 11410-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11410-1-AP (NAT2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 11410-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11410-1-AP (NAT2 Antibody) at dilution of 1:50 (under 40x lens).

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  FC experiment of MCF-7 using 11410-1-AP

  1x10^6 MCF-7 cells were intracellularly stained with 0.8 ug NAT2 Polyclonal antibody (11410-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.8 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

NAT2 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 11410-1-AP

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

N acetyltransferase type 2, EC:2.3.1.5, EC:2.3.1.118, Arylamine N-acetyltransferase 2, Arylamide acetylase 2

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Tested Applications

• Positive WB detected in: rat liver tissue
• Positive IHC detected in: human small intestine tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC (Intra) detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

11410-1-AP targets NAT2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NAT2 fusion protein Ag1953
• Full Name: N-acetyltransferase 2 (arylamine N-acetyltransferase)
• Calculated Molecular Weight: 33.5 kDa
• Observed Molecular Weight: 38 kDa
• GenBank Accession Number: BC015878
• Gene Symbol: NAT2
• Gene ID (NCBI): 10
• RRID: AB_3085371
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P11245
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NAT2 (Arylamine N-acetyltransferase 2) is well-known for its role in phase II metabolism of xenobiotics and drugs (PMID:36156098). NAT2 and its isozyme, arylamine N-acetyltransferase 1 (NAT1), play key roles in the detoxification of carcinogenic arylamines and xenobiotics by catalyzing acetyl-coenzyme A (CoA)-dependent biotransformation of arylamines to N-arylacetamides. Endogenous NAT2 expression is limited to the liver and small and large intestines, suggesting that NAT2 plays organ-specific roles (PMID:24467436).

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Hua (Verified Customer) (04-26-2024)

Very good antibody for WB. Strong signal and clean backgroud.

• Applications: Western Blot
• Primary Antibody Dilution: 1:4000
• Cell Tissue Type: Mouse liver

NAT2 Antibody Western Blot validation (1:4000 dilution) in Mouse liver (Cat no:11410-1-AP)