NAT10 Recombinant antibody, PBS Only

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  WB analysis using 82585-1-RR (same clone as 82585-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82585-1-RR (NAT10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82585-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 82585-1-RR (same clone as 82585-1-PBS)

  WB result of NAT10 antibody (82585-1-RR; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NAT10 transfected HeLa cells. This data was developed using the same antibody clone with 82585-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse testis using 82585-1-RR (same clone as 82585-1-PBS)

  IP result of anti-NAT10 (IP:82585-1-RR, 4ug; Detection:82585-1-RR 1:5000) with mouse testis tissue lysate 1120 ug. This data was developed using the same antibody clone with 82585-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 82585-1-RR (same clone as 82585-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NAT10 antibody (82585-1-RR, Clone: 5B9 ) at dilution of 1:1900 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 82585-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 82585-1-RR (same clone as 82585-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NAT10 antibody (82585-1-RR, Clone: 5B9 ) at dilution of 1:1900 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red). This data was developed using the same antibody clone with 82585-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82585-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82585-1-RR against Human NAT10 were performed. The affinity constant is below 1 pM.

• Featured Product
• KD/KO Validated

NAT10 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, IP, Indirect ELISA

Cat No. 82585-1-PBS

Clone No.5B9

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, IP, Indirect ELISA

Synonyms

N acetyltransferase 10, KIAA1709, EC:2.3.1.-, ALP, 5B9

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Product Information

82585-1-PBS targets NAT10 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: NAT10 fusion protein Ag4184
• Full Name: N-acetyltransferase 10 (GCN5-related)
• Calculated Molecular Weight: 1025 aa, 116 kDa
• Observed Molecular Weight: 116 kDa
• GenBank Accession Number: BC035558
• Gene Symbol: NAT10
• Gene ID (NCBI): 55226
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9H0A0
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

NAT10 (N-acetyltransferase 10) is a nucleolar protein that is involved in regulation of telomerase activity, DNA damage response, and cytokinesis. It also plays a role in maintaining nuclear shape. Inhibition of NAT10 has been reported to rescue the misshapen nuclei in laminopathic cells via microtubule reorganization. The specificity of this antibody has been tested by siRNA (PMID: 24786082). NAT10 regulates mitotic cell fate by acetylating Eg5. NAT10 depletion results in multinuclear giant cells, which is the hallmark of mitotic catastrophe (PMID: 35210604). NAT10 plays a crucial role in carcinogenesis through influencing EMT, hypoxia, ribosomal biogenesis and overall, promoting translational efficiency. Recently, we reported that treating cancer cells with Remodelin, a small molecule inhibitor of NAT10, causes alteration in global lipid metabolism (PMID: 36149760).