NAT1 Polyclonal antibody

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  WB analysis of A375 using 14394-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 14394-1-AP (NAT1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 14394-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 14394-1-AP (NAT1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 14394-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 14394-1-AP (NAT1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 14394-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 14394-1-AP (NAT1 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of A375 using 14394-1-AP

  Immunofluorescent analysis of A375 cells using 14394-1-AP (NAT1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

NAT1 Polyclonal Antibody for WB, IF, IHC, ELISA

Cat No. 14394-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Synonyms

AAC1, Arylamide acetylase 1, MNAT, N acetyltransferase type 1, NAT 1, NAT1, NATI

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Tested Applications

• Positive WB detected in: A375 cells, PC-3 cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A375 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

14394-1-AP targets NAT1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NAT1 fusion protein Ag5885
• Full Name: N-acetyltransferase 1 (arylamine N-acetyltransferase)
• Calculated Molecular Weight: 34 kDa
• Observed Molecular Weight: 32-34 kDa
• GenBank Accession Number: BC047666
• Gene Symbol: NAT1
• Gene ID (NCBI): 9
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P18440
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.