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NARS2 Polyclonal antibody

NARS2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 15714-1-AP

Synonyms

AsnRS, Asparagine tRNA ligase, NARS2, SLM5



Tested Applications

Positive WB detected inHepG2 cells, human liver tissue, human brain tissue, K-562 cells, mouse kidney tissue
Positive IHC detected inhuman kidney tissue, human heart tissue, human lung tissue, human placenta tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15714-1-AP targets NARS2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NARS2 fusion protein Ag8345
Full Name asparaginyl-tRNA synthetase 2, mitochondrial (putative)
Calculated Molecular Weight 477 aa, 54 kDa
Observed Molecular Weight 50-54 kDa
GenBank Accession NumberBC007800
Gene Symbol NARS2
Gene ID (NCBI) 79731
RRIDAB_2151157
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for NARS2 antibody 15714-1-APDownload protocol
IHC protocol for NARS2 antibody 15714-1-APDownload protocol
IF protocol for NARS2 antibody 15714-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols