NARFL-Specific Polyclonal antibody, PBS Only

NARFL-Specific Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 20209-1-PBS

Synonyms

---

Validation Data Gallery

• 

  WB analysis using 20209-1-AP (same clone as 20209-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 20209-1-AP (NARFL-Specific antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20209-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 20209-1-AP (same clone as 20209-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 20209-1-AP (NARFL-Specific antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20209-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 20209-1-AP (same clone as 20209-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 20209-1-AP (NARFL-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20209-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 20209-1-AP (same clone as 20209-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 20209-1-AP (NARFL-Specific antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 20209-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 20209-1-AP (same clone as 20209-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 20209-1-AP (NARFL-Specific antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 20209-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 20209-1-AP (same clone as 20209-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 20209-1-AP (NARFL-Specific antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 20209-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 20209-1-AP (same clone as 20209-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 20209-1-AP (NARFL-Specific antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 20209-1-PBS in a different storage buffer formulation.

---

Product Information

20209-1-PBS targets NARFL-Specific in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: nuclear prelamin A recognition factor-like
• Calculated Molecular Weight: 53 kDa
• Observed Molecular Weight: 26-28 kDa
• GenBank Accession Number: NM_022493
• Gene Symbol: NARFL
• Gene ID (NCBI): 64428
• RRID: AB_10694435
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

NARFL, also named as PRN and IOP1, belongs to the NARF family. NAFL is a component of the cytosolic iron-sulfur (Fe/S) protein assembly machinery. It is required for maturation of extramitochondrial Fe/S proteins. It seems to negatively regulate the level of HIF1A expression, although this effect could be indirect. [18270200], [16956324] The antibody is specific to NARFL.