NARF Polyclonal antibody, PBS Only

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  WB analysis of human liver using 12173-1-AP (same clone as 12173-1-PBS)

  human liver tissue were subjected to SDS PAGE followed by western blot with 12173-1-AP (NARF antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12173-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human placenta using 12173-1-AP (same clone as 12173-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 12173-1-AP (NARF antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12173-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse testis using 12173-1-AP (same clone as 12173-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12173-1-AP (NARF antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12173-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human lung using 12173-1-AP (same clone as 12173-1-PBS)

  human lung tissue were subjected to SDS PAGE followed by western blot with 12173-1-AP (NARF antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12173-1-PBS in a different storage buffer formulation.

NARF Polyclonal Antibody for WB, Indirect ELISA

Cat No. 12173-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

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Product Information

12173-1-PBS targets NARF in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NARF fusion protein Ag2816
• Full Name: nuclear prelamin A recognition factor
• Calculated Molecular Weight: 46 kDa, 53 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: BC016440
• Gene Symbol: NARF
• Gene ID (NCBI): 26502
• RRID: AB_2151027
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UHQ1
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

Prelamin A, is processed in the nucleus to lamin A by removal of its final 18 amino acids, including the cysteine residue in its C-terminal CAAX box, which is farnesylated. Nuclear prelamin A recognition factor (NARF) binds the farnesylated prelamin A C-terminal domain(preAct)(PMID:10514485). NARF, at 52 kDa, also named as IOP2, is a small enough protein to freely diffuse through the nuclear pore complex and may be retained within the nucleus by binding nuclear proteins and it has all of the information necessary to be imported into the nucleus(PMID:10514485). It has 3 isoforms produced by alternative splicing with the molecular mass of 51 kDa, 56 kDa and 46 kDa,respectively.