NAPRT1 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

NAPRT1 Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

5D8H10

Cat no : 66159-1-PBS

Synonyms

FHA HIT interacting protein, FHIP, NAPRT1, NAPRTase, PP3856

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Validation Data Gallery

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  WB analysis using 66159-1-Ig (same clone as 66159-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66159-1-Ig (NAPRT1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66159-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66159-1-Ig (same clone as 66159-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66159-1-Ig (NAPRT1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66159-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 cells using 66159-1-Ig (same clone as 66159-1-PBS)

  WB result of NAPRT1 antibody (66159-1-Ig, 1:6000) with si-Control and si-NAPRT1 transfected HepG2 cells. This data was developed using the same antibody clone with 66159-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66159-1-Ig (same clone as 66159-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66159-1-Ig (NAPRT1 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66159-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 66159-1-Ig (same clone as 66159-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66159-1-Ig (NAPRT1 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66159-1-PBS in a different storage buffer formulation.

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  IHC staining of human small intestine using 66159-1-Ig (same clone as 66159-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 66159-1-Ig (NAPRT1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66159-1-PBS in a different storage buffer formulation.

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  IHC staining of human small intestine using 66159-1-Ig (same clone as 66159-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 66159-1-Ig (NAPRT1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66159-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66159-1-Ig (same clone as 66159-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol ) fixed HeLa cells using 66159-1-Ig(NAPRT1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66159-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66159-1-Ig (same clone as 66159-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using NAPRT1 antibody (66159-1-Ig, Clone: 5D8H10 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66159-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66159-1-PBS targets NAPRT1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: NAPRT1 fusion protein Ag4265
• Full Name: nicotinate phosphoribosyltransferase domain containing 1
• Calculated Molecular Weight: 514 aa, 55 kDa
• Observed Molecular Weight: 51 kDa
• GenBank Accession Number: BC032466
• Gene Symbol: NAPRT1
• Gene ID (NCBI): 93100
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C.

Background Information

Nicotinic acid (NA) is a coenzyme in cellular redox reactions, and is an essential component of metabolic pathways in all living cells. NAPRT1 (Nicotinate phosphoribosyltransferase) is essential for increasing cellular NAD levels and, thus, to prevent oxidative stress of cells. NAPRT1 converts Nicotinic acid (NA; niacin) to NA mononucleotide (NaMN), which is then converted to NA adenine dinucleotide (NaAD), and finally to nicotinamide adenine dinucleotide (NAD).