NAPRT1 Polyclonal antibody

NAPRT1 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

16

Cat no : 13549-1-AP

Synonyms

FHA HIT interacting protein, FHIP, NAPRT1, NAPRTase, PP3856

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Validation Data Gallery

• 

  WB analysis using 13549-1-AP

  Mouse liver tissue were subjected to SDS PAGE followed by western blot with 13549-1-AP (NAPRT1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 13549-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 13549-1-AP (NAPRT1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human small intestine using 13549-1-AP

  human small intestine tissue were subjected to SDS PAGE followed by western blot with 13549-1-AP (NAPRT1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 13549-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 13549-1-AP (NAPRT1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 13549-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13549-1-AP (NAPRT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 13549-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 13549-1-AP (NAPRT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 13549-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 13549-1-AP (NAPRT1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 13549-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13549-1-AP (NAPRT1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human colon using 13549-1-AP

  human colon tissue were subjected to SDS PAGE followed by western blot with 13549-1-AP (NAPRT1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 13549-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13549-1-AP (NAPRT1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 13549-1-AP

  IP result of anti-NAPRT1 (IP:13549-1-AP, 4ug; Detection:13549-1-AP 1:1000) with HEK-293 cells lysate 2800ug.

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Tested Applications

• Positive WB detected in: mouse liver tissue, human colon tissue, HEK-293 cells, mouse kidney tissue, L02 cells, HepG2 cells
• Positive IP detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 15 publications below
• IHC: See 4 publications below
• IF: See 3 publications below

Product Information

13549-1-AP targets NAPRT1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NAPRT1 fusion protein Ag4265
• Full Name: nicotinate phosphoribosyltransferase domain containing 1
• Calculated Molecular Weight: 514 aa, 55 kDa
• Observed Molecular Weight: 51 kDa, 56 kDa
• GenBank Accession Number: BC032466
• Gene Symbol: NAPRT1
• Gene ID (NCBI): 93100
• RRID: AB_2151014
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Nicotinic acid (NA) is a coenzyme in cellular redox reactions, and is an essential component of metabolic pathways in all living cells. NAPRT1 (Nicotinate phosphoribosyltransferase) is essential for increasing cellular NAD levels and, thus, to prevent oxidative stress of cells. NAPRT1 converts Nicotinic acid (NA; niacin) to NA mononucleotide (NaMN), which is then converted to NA adenine dinucleotide (NaAD), and finally to nicotinamide adenine dinucleotide (NAD).

Publications

Species	Application	Title
human,mouse	WB	Cell Metab; NAD+ Metabolism Maintains Inducible PD-L1 Expression to Drive Tumor Immune Evasion.; Authors - Hongwei Lv
human	IF,WB	J Pharm Anal; Epigenetic drug library screening reveals targeting DOT1L abrogates NAD+ synthesis by reprogramming H3K79 methylation in uveal melanoma; Authors - Xiang Gu
	WB	Kidney Int; Anticystogenic activity of a small molecule PAK4 inhibitor may be a novel treatment for autosomal dominant polycystic kidney disease.; Authors - Vicki J Hwang
human	WB	Clin Cancer Res; Targeting of NAD metabolism in pancreatic cancer cells: potential novel therapy for pancreatic tumors.; Authors - Chini Claudia Cs CC
human,mouse	WB,IHC	Int J Mol Sci; NAD+ Anabolism Disturbance Causes Glomerular Mesangial Cell Injury in Diabetic Nephropathy.; Authors - Xue Li
human,mouse	IF	Metabolism; Hyperglycemia-reduced NAD+ biosynthesis impairs corneal epithelial wound healing in diabetic mice.; Authors - Ya Li